MRL research is supported by approximately 20,000 sq ft of BSL-2 laboratory space on CSU’s Main Campus (Microbiology Building and Pathology Building) and 10,000 sq ft of BSL3/ABSL3 laboratory space in state-of-the-art facilities on the Foothills Campus: the Infectious Disease Annex (IDA), the Regional Biocontainment Laboratory (RBL), and the Research Innovation Center (RIC) of the Infectious Disease Research Center (IDRC).
Autoclaves, biosafety cabinets, MilliQ water systems, ice machines, walk-in cold rooms, glass washing and drying equipment are standard in building infrastructure. In addition to the equipment available in each Faculty’s laboratory, the MRL shares numerous instruments, including incubators, environmental shakers, microscopes, vacuum pumps, centrifuges/ultracentrifuges, French pressure cell presses, scintillation counters, thermocycler, ELISA plate readers, lyophilizers, gas chromatographs, spectrophotometers, rotavapors, X-ray developers, HPLCs, and FPLCs.
BSL-3 Research Facilities
The Infectious Disease Research Center (IDRC), located at the CSU Foothills campus, is a 25,000 sq. ft. [BSL-2, BSL-3 and ABSL-3] facility with 9,100 sq. ft dedicated to BSL3 containment area. MRL laboratories have shared use of the Bulk Culture and Immunology BSL-3 Suites. The Bulk Culture Suite which is dedicated to TB research is approximately 3,000 sq ft and is utilized for the large-scale growth and manipulation of virulent M. tuberculosis including MDR-M. tb strains. This bulk culture/molecular biology suite contains all the equipment for culture manipulation (biosafety cabinets, 37°C warm rooms, 32°C and 39°C incubators, centrifuges, electroporator, pass-through autoclave, cold room).
The Immunology Suite in which cell and animal infections are carried out is equipped with a Calibar Flow Cytometer with a Macintosh computer loaded with Cell Quest software. Other equipment includes a real-time PCR detection system, a microplate fluorometer with PlateReader™ software, a Cytomation Mo-Flo MLS 2000 multi-laser cell sorter, a Beckman Coulter Z1 series particle counter, a Microplate ELISA reader, a ELISA microplate washer, a Leica Cryostat/Microtome for sectioning infected tissues for immuno-histochemical staining and microscopic analysis, and an Olympus BH2 microscope with RFCA reflected light fluorescence module with a Polaroid digital camera attachment. The Immunology suite also contains two Class II bio-safety cabinets for tissue culture, two CO2 incubators. Equipment within this laboratory includes: a Becton Dickinson FACSCalibur flow cytometer, The BDTM LSR II flow cytometer have the ability to detect up to 13 colors per sample. The BDTM LSR II flow cytometer is equipped with four lasers, in addition to a sophisticated BDTM High Throughput sampler that allow rapid collection of samples directly from a 96 well plates and the latest BD FACSDive software, a PE Biosystems ABI Prizm 7700 real-time PCR detection system.
MRL investigators also have shared use of a necropsy room which houses two class II bio-safety cabinets linked directly to class I bio-safety cabinets via an access door for the manipulation of infected tissues. Adjacent to this room is a dedicated area that houses 2 Glas-Col aerosol generating devices for exposure of experimental animals. Included in the IDRC is up to 8,000 sq ft of space for housing rodents under BSL3 and BSL2 containment. This facility has holding rooms for up to 2,000 mice and is equipped with Thoren racks, and HEPA-filtered cage changing stations. The IDRC facility also contains common rooms and facilities such as media prep and glassware washing rooms, a central office space with desks and computers, and locker and shower rooms. All procedures in the BSL-3 facility strictly adhere to CSU Biosafety Committee guidelines as well as those guidelines outlined in a Biosafety Procedure and Practice Manual.
Animal Care at CSU
The CSU animal care program is monitored through a single, central Institutional Animal Care and Use Committee (IACUC) that reports directly to the Vice President for Research. Lab Animal Resources (LAR) is an institutional core facility that oversees the care and management of animals used in research and teaching at Colorado State University in over 48,000 square feet of animal holding space for small and large animals including animal biosafety level 3. They collaborate with investigators to meet their research needs by providing a full-spectrum of animal care and related services.
The Painter Center is the primary, centralized animal facility located on Main Campus managed by LAR, that supports all aspects of animal-based research including those requiring Animal Biosafety Level 2 and 3 capabilities. The ABSL-3 laboratory is an isolated multi‑room suite of 692 sq ft. Investigators from 12 departments on campus, including MRL investigators, are served by this facility. Animals are housed under hygienic and humane conditions in quarters that are accredited by the American Association of Laboratory Animal Sciences.
Within the Infectious Disease Research Center (IDRC) facilities located on the Foothills Campus, there are multiple isolated suites housing animals in ABSL-2 and ABSL-3 conditions, one of them specifically dedicated to TB research.
CSU Veterinary Teaching Hospital
The CSU Veterinary Teaching Hospital is a nationally-recognized veterinary hospital, working to advance medicine by integrating world-class patient care with leadership in veterinary education and scientific discovery. The hospital admits all animal species, including more than 20,000 dogs and 3,500 cats per year. It is a full-service hospital catering to all specialties with services such as neurosurgery, endoscopy, diagnostic aids, MRI’s, CT’s and ultrasounds. The clinicians at the CSU VTH are specialists and serve as experts in their field.
Information Technology Infrastructure
All CSU facilities and labs are fully wired for information technology and all offices and laboratories contain multiple computers to provide the hardware and software necessary to do state-of-the-art science. Colorado State University fully supports the computers in these facilities with multiple full-time personnel.
Foundational Core Facilities
Foundational Core Facilities provide access to resources and/or services considered to be essential to the research enterprise. These facilities serve a broad spectrum of researchers spanning multiple departments and colleges within CSU as well as external academic and commercial partners and represent a center of excellence for a given technology or area of expertise. Foundational Core Facilities are financially supported, in part, by the OVPR to ensure stability and quality of these important resources.
The Proteomics & Metabolomics Facility (PMF) serves as an enabling resource for research and development programs at Colorado State University. They strive to build mass spectrometry instrumentation capabilities and expertise that exceed the normal resources of individual research programs, and make those technologies available as a shared resource. PMF also aims to provide an environment rich in expertise and educational resources, and to foster collaboration across the CSU community and beyond.
Proteomics services include protein identification, quantitative analysis of proteins and peptides (isobaric tagging, metabolic labeling, spectral counting, and selected reaction monitoring (SRM)), post-translational modification and protein digestion. Proteomics services are provided using the following instrumentation: Waters G2 TQ-S coupled with nanoUPLC, Bruker Microflex LRF, and Thermo Scientific Orbitrap Velos MS coupled with nanoHPLC. Mascot Database Search Enging, Scaffold, Scaffold Q+S, and Bruker Biotyper software are also available!
PMF offers both non-targed metabolite profiling (UPLC-MS, GC-MS) and targeted metabolite analysis (UPLC-MS, GC-MS, ICP-MS) of small molecules. Metabolomics services utilize the Waters G2 TOF coupled with UPLC, Waters G2 TOF coupled with UPLC, Elan DRC ICP-MS, two Waters G2 TQS systems coupled with microflow UPLC (IonKey Source), two Thermo Scientific Trace ISQ systems with liquid autosamplers, and the Thermo Scientific Trace ISQ with SPME and headspace autosampler instruments in the PMF laboratories. Provided analysis software include RAMclustR, Umetrics SimcaP (multivariate data analysis), and NIST MS SEARCH.
In addition to their provided services, PMF is able to offer the campus community a wide variety of research products from a number of vendors through the Life Science Stockroom and Enzyme Freezer Program. They maintain a stock of commonly used enzymes and molecular biology reagent kits, with negotiated preferred client discounts, haz-mat fee waivers, and free shipping for many special order items available from vendors.
The Central Instrument Facility (CIF) provides access to a large inventory of analytical instrumentation, expertise, and educational resources with a focus on chemical and material sciences and related disciplines. Many of the instruments in the CIF are available on a self-service basis with appropriate user training and advanced services are also available from CIF personnel. The CIF Is organized into 7 different laboratories, offering a complement of sophisticated instruments and technical expertise in the following categories: Imaging and Surface Science, Materials Analysis, Mass Spectrometry, Magnetic Resonance, Optical Spectroscopy, X-ray Spectroscopy and Diffraction, and the Bacterial and Mammalian Cell Culture Facility
Of particular interest is the Magnetic Resonance Laboratory which provides nuclear magnetic resonance (NMR) spectroscopy instruments, ranging from 300 to 600 MHz, consisting of:
- Agilent (Varian) 400MR equipped with Automated Tuning and a 7600 Sample Changer.
- Agilent Inova 400 equipped with proton detected multi-nuclear and quad-tuned probes. Extended VT range.
- Agilent Inova 300 equipped with quad-tuned (1H, 19F, 13C, 31P) probe. Extended VT ranged.
- Agilent Inova 500 with three channels, 3-axis gradients and many other accessories including HCN, broadband probes and a NANO (hrmas) probe.
- Agilent Inova 600 with four channels, 2H decoupling, 3-axis gradients and many other accessories including HCN and a flow-probe.
The Magnetics Resonance Laboratory also conducts electron paramagnetic resonance (EPR) spectroscopy, utilizing a Bruker ESR-300 spectrometer with various cavities for spin counting and very high temperature studies plus liquid helium cooled cavity for sub-10K studies
The Microscope Imaging Network (MIN) provides access to state of the art optical imaging systems across the CSU campus. User training and scheduling for various instruments is coordinated and expertise is available for experimental design consultation. The MIN includes the following:
- Zeiss LSM 510 Meta Laser Scanning Axiovert Confocal Microscope (W5 Anatomy-Zoology Building) has a META multi-spectral analyzer and a dual laser scanning system. Wavelengths available are 458, 488, 514, 543 and 633nm. It does not have a stage incubator and is used primarily for fixed samples unless the operator provides an incubation chamber. Zeiss imaging software is used to capture and process images.
- Zeiss LSM 510 Meta Laser Scanning Axiovert Confocal Microscope (B318 Microbiology Building) also has a META multi-spectral analyzer and a dual laser scanning system. Wavelengths available are 405, 458, 488, 514, 543 and 633nm. This instrument has a stage incubator for live cell imaging and Zeiss imaging software to capture and process images.
- Olympus IX81 Inverted Spinning Disk Confocal Microscope (224 Molecular & Radiological Sciences Building) has environmental control chamber with CO2 control for live cell work, an X,Y, piezo Z stage for rapid image stack acquisition across many predetermined fields (4 D imaging), a CSU 22 head with quad dichroic and additional emission filter wheel to eliminate spectral crossover, four high power diode lasers (405 nm, 488 nm, 561 nm and 647 nm) with rapid (microsecond) switcher and a phasor holographic photobleaching/photoactivation/photoconversion system for intracellular molecular dynamic measurements. Phasor can also be used for ion uncaging and other applications. System has differential interference contrast (DIC) optics with 10, 20, 40, 60 and 100X objectives, built in correction for spherical aberration for all objectives, and a wide field Xenon light source. A cascade II EMCCD camera (confocal imaging) and a Photometrics HQ camera (wide field imaging) are both integrated for image capture using Slidebook software. Laser selection and power are software controlled. System is connected to RAID terabyte storage.
- Olympus IX81 Inverted Confocal Laser Scanning Microscope (Research Innovation Center, Foothills Campus) has widefield fluorescence visualization, brightfield and differential interference contrast. Four channel spectral and filter-based confocal scanning includes a transmitted light channel for DIC. This feature provides sensitivity and flexibility for imaging with and without fluorescent fluorophores and will allow users to image up to four compatible fluorophores simultaneously. Objectives on this system are 10X, 20X, 40X, and 100X. More information about additional features.
- Nikon Total Internal Reflection Fluorescence (TIRF) Inverted Microscope (Eclipse Ti) (224 Molecular & Radiological Biosciences Building) has perfect focus control, a four laser line launch (405, 488, 561 and 640 nm) and an Intensilight Hg illumination source. A motorized XY Piezo Z stage and stage-top environmental chamber with temperature, CO2/air, humidity capability, will support culture dishes, multi-well plates or slides. Equipped with 20X, 40X, 60X DIC objectives and a 100X TIRF objective. Microscope has an Andor Clara camera for wide field imaging and an Andor iXon3 EMCCD camera for TIRF imaging. Microscope has built-in N-STORM super-resolution imaging capability and is networked to a RAID array for multiple terabyte storage. Nikon elements software controls all aspects of acquisition and analysis with a deconvolution software package included.
- 2 Nikon inverted epifluorescence Microscopes (224 Molecular & Radiological Biosciences building) with video cameras for digital imaging are available within the Fluorescence Microscopy/Image Analysis Center in room 224 MRB. Filter cubes for all common fluors (DAPI, Fluorescein, Rhodamine, Texas Red, Cy 3, Cy5) are available and are interchangeable between microscopes. One microscope has DIC optics, an optional phase oil-immersion condenser, and a stage incubator and filter wheel for live cell studies (ratio imaging included) and one has a color camera for imaging stained sections. A specialized light source for darkfield illumination of slides is available and is especially useful for counting silver grains on emulsion treated samples. All microscopes utilize Metamorph software for image capture and analysis.
- JEOL JEM-1400 120 keV Transmission Electron Microscope (W13 Anatomy & Zoology Building) is equipped with several different specimen holders (a single specimen holder, ±26°; a high tilt specimen holder, ±70°; a Fischione dual axis Tomography holder, ±70°; and a Gatan liquid nitrogen-cooled specimen holder, ±70°), an Orius fastscan digital camera, and a Gatan Ultrascan high-resolution digital camera. Direct magnification range on this TEM is between 50x and 1,200,000x.
- JEOL JEM-2000 EXII Transmission Electron Microscope (W13 Anatomy & Zoology Building) is equipped with a ±60° tilting (goniometer) stage, operates at accelerating voltages from 80kV to 200kV, and has a magnification range from 50x to 500,000x. This TEM is capable of selected area diffraction from 100 to 2,500mm. Two specimens can be loaded simultaneously. Images are currently captured on film, which is processed daily.
Emerging Innovations Core Facilities
Emerging Innovations Facilities provide access to resources and/or services in new or emerging area of research and technology. These facilities may serve a broad or focused spectrum of researchers primarily within CSU. Emerging Innovations Core Facilities may receive seed support to enable development and ensure agility of resources for new areas of research service.
The Flow Cytometry Facility (FCF) provides access to state of the art flow cytometry and cell sorting instrumentation across the CSU campus. User training and scheduling for various instruments is coordinated and expertise is available for experimental design consultation. The facility is co-directed by MRL faculty member, Marcela Henao Tamayo, and her co-director Christopher Allen. The Flow Cytometry Facility equipment includes:
- CyAn ADP 7 Color Analyzer (#1 in 479D Molecular & Radiological Biosciences building) has 9 parameters (FSC, SCC, FL1-FL7) and is equipped with 488 nm factory laser and a 640 nm, 90 mw ruby laser (adding 65 mw power over the factory supplied red laser). Assays using the red laser are the most robust of any CyAn in America.
- CyAN ADP 9 Color Analyzer (#2 in 479D Molecular & Radiological Biosciences building) is equipped with 405nm, 488 nm and 640 nm solid-state lasers and adds two fluorescent channels.
- HyperCyt Rapid Sampler for High Throughput Flow Cytometry (479D Molecular & Radiological Biosciences building) in use in conjunction with CyAn1 or CyAn2, rapidly acquires samples from a multi-well microplate to perform high-throughput flow cytometry. The HyperCyt System is designed to acquire samples in a variety of 96- and 384-well microplates. High-throughput sample acquisition is achieved by the HyperCyt Autosampler software module, which utilizes an innovative, patented sampling method. As the sampling probe of the autosampler moves from well to well in a multi-well microplate, a peristaltic pump aspirates sample particle suspensions from each well. Between each well the continuously running pump draws a gap of air into the sample line, resulting in the generation of a series of air-gap-separated samples for delivery to the flow cytometer. The time-resolved data, with periodic gaps corresponding to the passage of the sample-separating air gaps, are analyzed by the HyperView® Analysis software.
- Beckman Coulter Gallios Flow Cytometer (A106, Regional Biocontainment Laboratory (RBL), Foothills Campus) is a research system that delivers analytical excellence by coupling extraordinary sensitivity, resolution and dynamic range with high-speed data collection. Along with the unprecedented detection capabilities of the instrument, the Gallios includes easy-to-use software and automation to facilitate superior performance of multi-color flow cytometry assays (10 Colors, 3 lasers – 405, 488 and 638 nm).
- BD LSR II Flow Cytometer (Biohazard Research Building (BRB) BSL3 , Foothills Campus) with the ability to detect up to 10 colors per sample. The BD LSR II flow cytometer is equipped with four lasers (355, 405, 488 and 633 nm) in addition to a sophisticated BD High Throughput sampler that allow rapid collection of samples directly from a 96-well plates and the latest BD FACSDiva software.
- BD FACSAria-III (?? Molecular & Radiological Biosciences Building (MRB)) delivers proven multicolor performance: 6 color-based cell sorting, two lasers (488 and 633 mm). Its fluidics and optical systems include unique design innovations precisely integrated to maximize signal detection. These innovations include the laser excitation optics, the patented flow cell with gel-coupled cuvette, and the highly efficient, patented octagon and trigon modules. Together, these systems allow theFACSAria III to achieve unrivaled sensitivity and resolution.
- MoFlo Flow Cytometer and High Speed Cell Sorter (101B Pathology Building) has three colored lasers (solid state iCyt 488nm blue laser, 635nm red diode laser, coherent radius 405nm violet laser) and is capable of measuring up to 9 fluorescent parameters in addition to forward and side light scatter. It can sort as many as 4 sub-populations of cells at one time, rather than the standard 2 fractions. Using the automated Cyclone attachment, the MoFlo can sort single or any specified number of cells into 96 well plates, though other sizes of plates, trays and microscope slides can be used. Due to the complexity of this instrument, all analysis and cell sorting experiments need to be scheduled with the trained operator. The purity and yield of the sort can be maximized according to client needs.
The Experimental Pathology Facility (EPF) operates through state-of-the-art histology and bioanalytical laboratories located within Veterinary Diagnostic Laboratories and is directed by MRL faculty member, Brendan Podell. The EPF has three primary objectives: to provide specialized pathology consultation and collaboration for researchers performing basic science research and preclinical studies, both within and outside of Colorado State University, to provide expert histological processing of research samples, interpretation of disease morphology, and quantitative methods for determining disease severity, pathogenesis and treatment efficacy, and to provide expert laboratory services and interpretation for hematology, clinical chemistry, and cytology.
Anatomic histology services include preparation of tissue specimens for histopathological review with Paraffin block embedding, microtome sectioning, frozen tissue sectioning, H&E staining using automated stainer and coverslipping instruments, and a multitude of alternative histochemical stains. Development and implementation of immunological detection methods in tissue and cells using automated immunostaining instruments such as immunohistochemistry, immunocytochemistry, in situ hybridization, and immunofluorescence.
Advanced digital pathology services provide bright field and fluorescent digital slide scanning, quantitative image analysis techniques for immunofluorescence and immunohistochemistry, and quantitative area morphometry and stereology techniques. Clinical pathology services are grouped into the following categories: hematology with complete blood count and coagulation testing, bench chemistry (urinalysis, immunohematology, fluid analysis), automated serum chemistry (diagnostic panels and blood gas analysis) and cytology (fine needle aspirates, impression smears, immunocytochemistry panels).
The Molecular Quantification Core provides access and training for molecular quantification tools including state-of-the-art BioRad QX200 Digital Droplet PCR System and a GE Healthcare Typhoon Trio Imager. The full QX200 Digital Droplet PCR System includes the droplet generator, plate sealer, C1000 PCR machine plus droplet reader B406 of the Microbiology building. Three satellite droplet generation/PCR stations are located around Main and Foothills Campus for widespread campus sample preparation.
The Typhoon Trio Imager is located in B319 of the Microbiology building and supports fluorescent imaging through three lasers enabling excitation and visualization of a wide range of wavelengths. Applications include fluorescent stained protein gels (SYPRO Ruby, Deep Purple), fluorescent western blots (ECL Plus, ECL Plex), fluorescent nucleic acid stains (SYBR-green), 2D-DIGE (Cy2, Cy3, Cy5) etc. The Typhoon is not recommended for straight chemiluminescence, but fluorescent alternatives such as ECL Plus, and Cy-dye labeled secondary antibodies do allow western blotting experiments to be analyzed. Analysis is performed using ImageQuantTL software which is loaded on the Typhoon computer.
Specialized Research Service Facilities
Specialized Research Service Facilities provide access to resources and/or services within a unit or research community. These facilities may receive one-time funding support from the OVPR to enable specific activities but are typically supported through their home unit.
The Next Generation Sequencing Core Facility operates a self-service facility to enable genomics research at CSU by providing access to sequencing instruments, an library preparation laboratory, and other instruments required for sample processing, library prep, library quantification and QC. Facility staff offer support and training in the use of sequencing instruments as well as running libraries and performing pre-run library QC (qPCR-based library quantification and tapestation analysis) when requested.
The facility houses an Illumina MiSeq and an Illumina NextSeq 500 next generation sequences as well as the following instruments: Covaris S2 sonicator, Agilent 2100 bioanalyzer, Agilent tapestation 2200, ABI GeneAmp 9700 thermocyclers, ABI StepOnePlus qPCR instrument, Qubit 2.0 fluorometer, Nanodrop 2000 spectrophotometer, Thermo Savant DNA120 SpeedVac Concentrator, and Invitrogen E-gel apparatus. The open-access dedicated NGS laboratory also has standard equipment such as vortexes, centrifuges, and pipettes. Though certain consumables and reagants are available
The Chemistry Stockroom located in the Chemistry Building D-wing, provides a large selection of chemicals and scientific supplies to the university, without the necessity of each college, department or research group maintaining its own separate storeroom. The Chemistry Stockroom is not open to the public, but provides an easy one-stop shop method for campus members.
The Chemistry Department also supports an electronics shop, a glassblowing shop and a machine shop to support campus-wide research efforts with full-time expert personnel, who can design, construct and repair existing and custom components/systems.
The CSU Veterinary Diagnostic Laboratory (VDL) is composed of a main laboratory location at South Campus as well as branch laboratories in Grand Junction and Rocky Ford, Colorado. All of the laboratories are accredited by the American Association of Veterinary Laboratory Diagnosticians and recently, the Fort Collins and Rocky Ford Veterinary Diagnostic Laboratories were recognized as approved laboratories in the National Animal Health Laboratory Network (NAHLN). The mission is to provide timely, accurate, and pertinent animal disease diagnostic services to veterinarians, their clients, animal industries, and those responsible for the detection and prevention of disease in animals and diseases affecting public health.
As such, the CSU VDL also contributes to research through new approaches to disease identification, investigation, and prevention; sharing scientific information through scholarly publication and outreach; and contributing to the education of professional veterinary medical, graduate, undergraduate, and post-doctoral students. The CSU VDL is made up of a multidisciplinary faculty and staff representing specialists in various areas of expertise, including pathologists, bacteriologists, virologists, toxicologists, parasitologists, histotechnologists, bringing a breadth and depth to the diagnostic medicine services available. . The CSU VDL is organized into the following focus areas: Avian Diagnostics, Bactriology, Chemistry/Toxicology, Clinical Immunology, Histopathology, Immunohistochemistry, Molecular Diagnostics, Parasitology, Pathology, Specialized Infectious Disease, Transmissible Spongiform Encephalopathies (TSE) and Virology.
The Histone Source strives to provide top quality histones, chromatin, and other custom proteins for research. They regularly analyze their products for stringent quality control such that they end up with exactly what they need for specified research projects. The Protein Expression and Purification Facility at The Histone Source has advanced equipment and access to state of the art mass spectrum instruments to provide a protein product that has been analyzed for quantity and purity before shipment. From clone to construct, they can produce difficult to purify protein products in a short time frame and their work is guaranteed.
Other Research Facilities
The Protein Purification and Characterization Facility is dedicated to the fabrication, purification, solution-state characterization and crystal structure determination of proteins. The specialized facility offers instrumentation, training, consulting and assistance to the research community, both at CSU and the external scientific community. The Protein Purification and Characterization is made up of three smaller facilities including:
- Solutions Characterization Facility provides access to analytical ultracentrifugation & circular dichroism spectroscopy for solution-state analysis of proteins, nucleic acids and macromolecular complexes.
- Histone Source, Protein Expression and Purification Facility (see Specialized Research Service Facilities.)
- Marcomolecular X-ray Crystallography Facility includes a temperature-controlled crystallization room, rotating anode x-ray generator, two image plate detectors, and a computational facility for elucidating protein and nucleic acid structure at atomic resolution
The Irradiation Services Laboratory provides accurate, convenient and reliable access to ionizing radiation to CSU and external researchers. Irradiations include molecular compounds, cell cultures, animal and human cells, bacteria and whole and partial body irradiation for small-animal studies. The laboratory’s irradiators are found in several locations in the Molecular and Radiological Biosciences (MRB) building and can be configured to support a diverse range of research applications and goals. Equipment currently available includes five cesium irradiators, one cobalt irradiator, and an X-ray machine.
The Fluorescence Microscopy/Image Analysis Center (FMIAC) is housed in Molecular & Radiological Biosciences Building. It consists of equipment for obtaining digitized images of gels, blots, and microtiter plates for radioactivity, chemiluminescence and chemifluorescence in the Macroscopic Imaging Facility and for various light/fluorescence microscopy applications in the Microscopic Imaging facility. The Microscopic Imaging Facility instruments are part of the CSU Microscope Imaging Network (see Foundational Core Facilities for more information).
The Macroscopic Imaging Center contains a Typhoon FLA 9500 for radioisotopic, chemiluminescence and fluorescence imaging. Laser lines of 473, 532, 635, 685 and 785 are available. Large format phosphorimager screens allow detection of radioactivity over a 105 dynamic range. The Macroscopic Imaging Center facility also contain an ImageQuant LAS 500 detection system for chemiluminescence, fluorescence and colorometric measurements from microtiter format plates and a LiCor Odyssey CLx near IR Scanner for dual color Western blot analysis.